Jump to content

microbial growth and C,N and P mass conservation


T.C. Onstott
 Share

Recommended Posts

Dear Tom,

 

I would like to grow biomass using the microbe reaction and monitor depletion of critical carbon substrate, N (i.e. NH3(a) and P (HPO4--) to determine when an environment becomes substrate limited as opposed to energy limited. I am not sure how to do this.

I created a mineral phase bioacetate(s) with the formula C36O40H102N9P1 and comprised of 4 species, Acetic_acid(aq), NH3(aq), HPO4-- and H+. this work fine. I then have a microbe reaction that uses Acetic_acid(aq) and calculates biomass. What I then tried to do is to create a custom rate law for C36O40H102N9P1 where the rate is equal to (biomass-biomass0)/Deltat. I thought that the custom rate laws could import these values like they do in the ratelaw_custom4.bas example, but no luck. I just get a can't evaluate the rate error message. Before I beat my head against a wall, I thought I would check in to see if you can offer some advice. If my code reads as follows:

 

kinetic microbe-24 rxn = "Acetate + H2O -> Methane(aq) + HCO3-" rate_law = ratelaw_example5.bas rate_con = 9e-10

kinetic microbe-24 biomass = 2e-14

kinetic microbe-24 growth_yield = 1

kinetic microbe-24 ATP_energy = -6

kinetic microbe-24 ATP_number = 1

kinetic microbe-24 Decay_con = 1e-11

kinetic microbe-24 power(Acetate) = 1

kinetic microbe-24 powerD(Acetate) = 1

kinetic microbe-24 order1 = 1

kinetic microbe-24 order2 = 1

kinetic microbe-24 KD = 8.6e-4

 

react 0.1 grams of "bioacetate(s)"

kinetic "bioacetate(s)" rate_law = ratelaw_bioacetate2.bas rate_con = 3.18e-8 surface = 1

 

 

do I need to explicitly state something like

 

kinetic "bioacetate(s)" biomass0 = ???

kinetic "bioacetate(s)" biomass = ???

 

in order for this ratelaw to be evaluated? Ideally I want to use the same biomass given by microbe-24.

 

Thanks, tco

Link to comment
Share on other sites

Hi T.C.:

 

The problem is that both reactants, the microbial population and your bioacetate mineral, have their own biomass and biomass0 variable. The only way to have one reactant set the value of the 2 variables for the other would be to write a C++ function, and link it into a DLL.

 

I hope that helps,

 

Tom Meuzelaar

RockWare, Inc.

Link to comment
Share on other sites

Join the conversation

You can post now and register later. If you have an account, sign in now to post with your account.

Guest
Reply to this topic...

×   Pasted as rich text.   Paste as plain text instead

  Only 75 emoji are allowed.

×   Your link has been automatically embedded.   Display as a link instead

×   Your previous content has been restored.   Clear editor

×   You cannot paste images directly. Upload or insert images from URL.

Loading...
 Share

×
×
  • Create New...